Antibody was affinity purified using an epitope specific to MOF/MYST1 immobilized on solid support. The epitope recognized by A300-994A maps to a region between residue 100 and 150 of human males absent on the first (MYST histone acetyltransferase 1) using the numbering given in entry BAB13924.1 (GeneID 84148). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
histone acetyltransferase KAT8, histone acetyltransferase MYST1, hMOF, K(lysine) acetyltransferase 8, Lysine acetyltransferase 8, MOF, MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1, MYST histone acetyltransferase 1, MYST1, MYST-1, ortholog of Drosophila males
between 100 and 150
2 - 8°C
Detection of human MOF/MYST1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and Jurkat cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody A300-994A (lot A300-994A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.