Antibody was affinity purified using an epitope specific to MOF/MYST1 immobilized on solid support. The epitope recognized by A300-992A maps to a region between residue 1 and 50 of human males absent on the first (MYST histone acetyltransferase 1) using the numbering given in entry BAB13924.1 (GeneID 84148). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
histone acetyltransferase KAT8, histone acetyltransferase MYST1, hMOF, K(lysine) acetyltransferase 8, Lysine acetyltransferase 8, MOF, MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1, MYST histone acetyltransferase 1, MYST1, MYST-1, ortholog of Drosophila males
between 1 and 50
2 - 8°C
Detection of human MOF/MYST1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody A300-992A (lot A300-992A-2) used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). MOF/MYST1 was also immunoprecipitated by a previous lot (lot A300-992A-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).