Antibody was affinity purified using an epitope specific to MKRN1 immobilized on solid support. The epitope recognized by A300-990A maps to a region between residue 432 and the C-terminus (residue 482) of human makorin, ring finger protein, 1 using the numbering given in entry NP_038474.1 (GeneID 23608). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
E3 ubiquitin-protein ligase makorin-1, RING finger protein 61, RING-type E3 ubiquitin transferase makorin-1, RNF61
between 432 and C-term
2 - 8°C
Detection of human MKRN1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-MKRN1 antibody A300-990A used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human MKRN1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- MKRN1 (Cat. No. A300-990A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB