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Rabbit anti-HRS Antibody, Affinity Purified - A300-989A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-HRS Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-989A
Name:
Rabbit anti-HRS Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to HRS immobilized on solid support.The epitope recognized by A300-989A maps to a region between residue 727 and the C-terminus (residue 777) of human growth factor-regulated tyrosine kinase substrate using the numbering given in entry NP_004703.1 (GeneID 9146).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
hepatocyte growth factor-regulated tyrosine kinase substrate, HRS, human growth factor-regulated tyrosine kinase substrate, protein pp110
Species Reactivity:
Human, Mouse
Immunogen:
Between 727 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human HRS by western blot. Samples: Whole cell lysate (50, 15, 5 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody A300-989A (lot A300-989A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of mouse HRS by western blot. Samples: Whole cell lysate (15 µg) from Renca, CT26.WT and 4T1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody A300-989A (lot A300-989A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human HRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HRS antibody A300-989A (lot A300-989A-2) used for IP at 6 µg per reaction. HRS was also immunoprecipitated by a previous lot of this antibody (A300-989A-1) and rabbit anti-HRS antibody A300-988A. For blotting immunoprecipitated HRS, A300-989A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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