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Rabbit anti-HRS Antibody, Affinity Purified

Catalogue number:
A300-989A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to HRS immobilized on solid support. The epitope recognized by A300-989A maps to a region between residue 727 and the C-terminus (residue 777) of human growth factor-regulated tyrosine kinase substrate using the numbering given in entry NP_004703.1 (GeneID 9146). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

hepatocyte growth factor-regulated tyrosine kinase substrate, HRS, human growth factor-regulated tyrosine kinase substrate, protein pp110

Species Reactivity:

Human, Mouse

Immunogen:

between 727 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human HRS by western blot.

Detection of human HRS by western blot. Samples: Whole cell lysate (50, 15, 5 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody A300-989A (lot A300-989A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse HRS by western blot.

Detection of mouse HRS by western blot. Samples: Whole cell lysate (15 µg) from Renca, CT26.WT and 4T1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody A300-989A (lot A300-989A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human HRS by western blot o

Detection of human HRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HRS antibody A300-989A (lot A300-989A-2) used for IP at 6 µg per reaction. HRS was also immunoprecipitated by a previous lot of this antibody (A300-989A-1) and rabbit anti-HRS antibody A300-988A. For blotting immunoprecipitated HRS, A300-989A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.