Antibody was affinity purified using an epitope specific to CTNND1 immobilized on solid support. The epitope recognized by A300-977A maps to a region between residue 625 and 675 of human catenin (cadherin-associated protein), delta 1 using the numbering given in entry NP_001322.1 (GeneID 1500). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BCDS2, cadherin-associated Src substrate, CAS, catenin (cadherin-associated protein), delta 1, catenin delta-1, CTNND, p120, p120 catenin, p120(CAS), p120(CTN), P120CAS, P120CTN
between 625 and 675
2 - 8°C
Detection of human CTNND1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CTNND1 antibody A300-977A used for IP at 3 µg/mg lysate. CTNND1 was also immunoprecipitated by rabbit anti-CTNND1 antibodies A300-976A and A300-978A, which recognize other epitopes. For blotting immunoprecipitated CTNND1, A300-978A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.