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Rabbit anti-ANKRD28/PITK Antibody, Affinity Purified - A300-974A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-ANKRD28/PITK Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-974A
Name:
Rabbit anti-ANKRD28/PITK Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to ANKRD28/PITK immobilized on solid support.The epitope recognized by A300-974A maps to a region between residue 975 and 1025 of human ankyrin repeat domain 28 (phosphatase interactor targeting K protein) using the numbering given in entry NP_056014.2 (GeneID 23243).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ankyrin repeat domain-containing protein 28, phosphatase interactor targeting K protein, phosphatase interactor targeting protein hnRNP K, PITK, PP6-ARS-A, PPP1R65, protein phosphatase 1, regulatory subunit 65, protein phosphatase 6 ankyrin repeat subunit
Species Reactivity:
Human
Immunogen:
Between 975 and 1025
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human ANKRD28/PITK by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa cells (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ANKRD28/PITK antibody A300-974A used for WB at 0.02 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). ANKRD28/PITK was also immunoprecipitated by rabbit anti-ANKRD28/PITK antibody BL4320, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

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Detection of human ANKRD28/PITK by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ANKRD28/PITK (Cat. No. A300-974A Lot1) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB

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