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Rabbit anti-FALZ/BPTF Antibody, Affinity Purified - A300-973A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-FALZ/BPTF Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-973A
Name:
Rabbit anti-FALZ/BPTF Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to FALZ/BPTF immobilized on solid support.The epitope recognized by A300-973A maps to a region between residue 1350 and 1400 of human Fetal Alzheimer Antigen (Bromodomain and PHD Domain Transcription Factor) using the numbering given in entry NP_872579.2 (GeneID 2186).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
bromodomain and PHD domain transcription factor, bromodomain and PHD finger-containing transcription factor, FAC1, FALZ, fetal Alz-50 clone 1 protein, fetal Alz-50 reactive clone 1, fetal Alzheimer antigen, NEDDFL, nucleosome remodeling factor, large subu
Species Reactivity:
Human
Immunogen:
Between 1350 and 1400
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human FALZ/BPTF by western blot. Samples: Whole cell lysate (15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FALZ/BPTF antibody A300-973A (lot A300-973A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human FALZ/BPTF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FALZ/BPTF antibody A300-973A (lot A300-973A-2) used for IP at 6 µg per reaction. For blotting immunoprecipitated FALZ/BPTF, A300-973A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human FALZ/BPTF by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FALZ/BPTF Cat. No. A300-973A Lot1 used at a dilution of 1:250 (0.8µg/ml) (left) and Cat. No. A300-973A Lot2 used at a dilution of 1:1,000 (0.2µg/ml) (right). Detection: DAB

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