Antibody was affinity purified using an epitope specific to SAPS3 immobilized on solid support. The epitope recognized by A300-972A maps to a region between residue 743 and the C-terminus (residue 793) of human SAPS domain family, member 3 using the numbering given in entry NP_060782.2 (GeneID 55291). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C11orf23, PP6R3, SAP190, SAPL, SAPLa, SAPS domain family member 3, SAPS domain family, member 3, SAPS3, serine/threonine-protein phosphatase 6 regulatory subunit 3, sporulation-induced transcript 4-associated protein SAPL
between 743 and C-term
2 - 8°C
Detection of human SAPS3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa cells (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SAPS3 antibody A300-972A used for WB at 0.02 µg/ml (A) and 0.04 µg/ml (B) and used for IP at 3 µg/mg lysate (B). SAPS3 was also immunoprecipitated by rabbit anti-SAPS3 antibody A300-971A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).