Antibody was affinity purified using an epitope specific to SAPS3 immobilized on solid support. The epitope recognized by A300-971A maps to a region between residue 400 and 450 of human SAPS domain family, member 3 using the numbering given in entry NP_060782.2 (GeneID 55291). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C11orf23, PP6R3, SAP190, SAPL, SAPLa, SAPS domain family member 3, SAPS domain family, member 3, SAPS3, serine/threonine-protein phosphatase 6 regulatory subunit 3, sporulation-induced transcript 4-associated protein SAPL
between 400 and 450
2 - 8°C
Detection of human SAPS3 by western blot of immunoprecipitates. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SAPS3 antibody A300-971A used for IP at 3 µg/mg lysate. SAPS3 was also immunoprecipitated by rabbit anti-SAPS3 antibody A300-972A, which recognizes a downstream epitope. For blotting immunoprecipitated SAPS3, A300-972A was used at 0.02 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.