Antibody was affinity purified using an epitope specific to SAPS2 immobilized on solid support. The epitope recognized by A300-969A maps to a region between residue 775 and 825 of human SAPS domain family, member 2 using the numbering given in entry NP_055493.2 (GeneID 9701). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
KIAA0685, PP6R2, SAP190, SAPS domain family member 2, SAPS domain family, member 2, SAPS2, serine/threonine-protein phosphatase 6 regulatory subunit 2
between 775 and 825
2 - 8°C
Detection of human SAPS2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SAPS2 antibody A300-969A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). SAPS2 was also immunoprecipitated by rabbit anti-SAPS2 antibody A300-970A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).