Antibody was affinity purified using an epitope specific to PPP2R3B immobilized on solid support. The epitope recognized by A300-965A maps to a region between residue 300 and 350 of human protein phosphatase 2 (formerly 2A), regulatory subunit B'', beta using the numbering given in entry NP_037371.2 (GeneID 28227). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: IP
NYREN8, NY-REN-8 antigen, PP2A subunit B isoform PR48, PP2A, subunit B, PR48 isoform, PPP2R3L, PPP2R3LY, PR48, PR70, protein phosphatase 2 (formerly 2A), regulatory subunit B'', beta, protein phosphatase 2, regulatory subunit B'', beta, Protein phosphatas
between 300 and 350
2 - 8°C
Detection of human PPP2R3B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PPP2R3B antibody A300-965A used for IP at 3 µg/mg lysate. PPP2R3B was also immunoprecipitated by rabbit anti-PPP2R3B antibodies A300-966A and BL4269, which recognize downstream epitopes. For blotting immunoprecipitated PPP2R3B, A300-966A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.