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Highly effective & efficient in vitro delivery of Cas9 RNP for genome editing in challenging cells

Rabbit anti-PPP2R2B Antibody, Affinity Purified - A300-964A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-PPP2R2B Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-964A
Name:
Rabbit anti-PPP2R2B Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to PPP2R2B immobilized on solid support.The epitope recognized by A300-964A maps to a region between residue 25 and 75 of human protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), beta isoform using the numbering given in entry NP_004567.1 (GeneID 5521).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
B55BETA, PP2A subunit B isoform B55-beta, PP2A subunit B isoform beta, PP2A subunit B isoform PR55-beta, PP2A subunit B isoform R2-beta, PP2A, subunit B, B-beta isoform, PP2AB55BETA, PP2ABBETA, PP2APR55B, PP2APR55BETA, PR2AB55BETA, PR2ABBETA, PR2APR55BETA
Species Reactivity:
Human, Mouse
Immunogen:
Between 25 and 75
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse PPP2R2B by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP2R2B antibody A300-964A (lot A300-964A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human PPP2R2B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP2R2B antibody A300-964A (lot A300-964A-2) used for IP at 6 µg per reaction. PPP2R2B was also immunoprecipitated by rabbit anti-PPP2R2B antibody BL4250. For blotting immunoprecipitated PPP2R2B, A300-964A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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