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Rabbit anti-Timeless Antibody, Affinity Purified

Catalogue number:
A300-960A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Timeless immobilized on solid support. The epitope recognized by A300-960A maps to a region between residue 1120 and 1170 of human Timeless using the numbering given in entry NP_003911.1 (GeneID 8914). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP
Product Type:

Antibodies

Alternative Names:

hTIM, protein timeless homolog, TIM, TIM1, timeless circadian clock 1, timeless homolog, Tof1 homolog

Species Reactivity:

Human

Immunogen:

between 1120 and 1170

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Timeless by western b

Detection of human Timeless by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Timeless antibody A300-960A (lot A300-960A- 2 ) used for IP at 6 µg per reaction. Timeless was also immunoprecipitated by rabbit anti-Timeless antibody A300-961A. For blotting immunoprecipitated Timeless, A300-960A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.