Antibody was affinity purified using an epitope specific to THRAP3/TRAP150 immobilized on solid support. The epitope recognized by A300-955A maps to a region between residue 375 and 425 of human Thyroid Hormone Receptor Associated Protein 3 (Thyroid Hormone Receptor-Asscociated Protein, 150 kDa) using the numbering given in entry NP_005110.1 (GeneID 9967). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BCLAF1 and THRAP3 family member 2, BCLAF2, thyroid hormone receptor-associated protein 3, thyroid hormone receptor-associated protein complex 150 kDa component, thyroid hormone receptor-associated protein, 150 kDa subunit, TRAP150, TR-associated protein,
between 375 and 425
2 - 8°C
Detection of human THRAP3/TRAP150 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-THRAP3/TRAP150 antibody A300-955A used for IP at 3 µg/mg lysate. THRAP3/TRAP150 was also immunoprecipitated by rabbit anti-THRAP3/TRAP150 antibodies BL4215 and A300-956A, which recognize downstream epitopes. For blotting immunoprecipitated THRAP3/TRAP150, A300-956A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.