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Rabbit anti-DOT1L Antibody, Affinity Purified - A300-953A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-DOT1L Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-953A
Name:
Rabbit anti-DOT1L Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to DOT1L immobilized on solid support.The epitope recognized by A300-953A maps to a region between residue1000 and 1050 of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC, ChIP-Seq

Product Type:
Antibodies
Alternative Names:
DOT1, DOT1 like histone H3K79 methyltransferase, DOT1-like histone methyltransferase, DOT1-like protein, DOT1-like, histone H3 methyltransferase, H3-K79-HMTase, histone H3-K79 methyltransferase, histone methyltransferase DOT1L, histone-lysine N-methyltran
Species Reactivity:
Human
Immunogen:
Between 1000 and 1050
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human DOT1L by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody A300-953A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). DOT1L was also immunoprecipitated by rabbit anti-DOT1L antibody A300-954A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

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Detection of human DOT1L by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L (Cat. No. A300-953A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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Localization of DOT1L Binding Sites by ChIP-sequencing. Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody A300-953A and analyzed by DNA sequencing. The figure illustrates the peak distribution of DOT1L binding within a 250 Kb region of chromosome 10 as detected using anti-DOT1L antibody A300-953A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.

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