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Rabbit anti-DOT1L Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (200 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Fri 5 Apr
Shipping is calculated in checkout
Applications:

WB, IP, IHC, ChIP-Seq

Antibody Host:

Rabbit

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 1000 and 1050

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Product Description:

Rabbit anti-DOT1L Antibody, Affinity Purified - 100 µl (200 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human DOT1L by western blot
Detection of human DOT1L by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody A300-953A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). DOT1L was also immunoprecipitated by rabbit anti-DOT1L antibody A300-954A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Detection of human DOT1L by immunohistoc
Detection of human DOT1L by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L (Cat. No. A300-953A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB
Localization of DOT1L Binding Sites by C
Localization of DOT1L Binding Sites by ChIP-sequencing. Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody A300-953A and analyzed by DNA sequencing. The figure illustrates the peak distribution of DOT1L binding within a 250 Kb region of chromosome 10 as detected using anti-DOT1L antibody A300-953A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.
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