Antibody was affinity purified using an epitope specific to TCEB3 immobilized on solid support. The epitope recognized by A300-942A maps to a region between residue 325 and 375 of human Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, elongin A) using the numbering given in entry NP_003189.1 (GeneID 6924). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
EloA, elongin 110 kDa subunit, elongin-A, RNA polymerase II transcription factor SIII subunit A1, SIII, SIII p110, TCEB3, TCEB3A, transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A), transcription elongation factor B alpha subunit,
between 325 and 375
2 - 8°C
Detection of human TCEB3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TCEB3 antibody A300-942A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). TCEB3 was also immunoprecipitated by rabbit anti-TCEB3 antibodies A300-943A and BL4209, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).