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Antibodies for Immuno-Oncology Research

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Rabbit anti-SPTBN1 Antibody, Affinity Purified - A300-936A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-SPTBN1 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-936A
Name:
Rabbit anti-SPTBN1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to SPTBN1 immobilized on solid support.The epitope recognized by A300-936A maps to a region between residue 2100 and 2150 of human Spectrin Non-Erythroid Beta 1 using the numbering given in entry NP_003119.2 (GeneID 6711).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
beta-fodrin, beta-G spectrin, beta-II spectrin, beta-spectrin 2, beta-spectrin II, beta-spectrin non-erythrocytic 1, betaSpII, ELF, embryonic liver beta-fodrin, epididymis luminal protein 102, fodrin beta chain, HEL102, spectrin beta chain, brain 1, spect
Species Reactivity:
Human, Mouse
Immunogen:
Between 2100 and 2150
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse SPTBN1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SPTBN1 antibody A300-936A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). SPTBN1 was also immunoprecipitated by rabbit anti-SPTBN1 antibody A300-937A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).

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Detection of human SPTBN1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SPTBN1 (Cat. No. A300-936A Lot1) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB

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