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Rabbit anti-Geminin Antibody, Affinity Purified - A300-935A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Geminin Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-935A
Name:
Rabbit anti-Geminin Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Geminin immobilized on solid support.The epitope recognized by A300-935A maps to a region between residue 160 and the C-terminus (residue 209) of human Geminin DNA replication Inhibitor using the numbering given in entry NP_056979.1 (GeneID 51053).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
Gem, geminin, MGORS6
Species Reactivity:
Human
Immunogen:
Between 160 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Geminin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Geminin antibody A300-935A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Geminin was also immunoprecipitated by rabbit anti-Geminin antibodies BL4159 and A300-934A, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 10 minutes (A) and 30 seconds (B).

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Detection of human Geminin by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin (Cat. No. A300-935A Lot1) used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB

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