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Rabbit anti-Geminin Antibody, Affinity Purified - A300-934A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Geminin Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-934A
Name:
Rabbit anti-Geminin Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Geminin immobilized on solid support.The epitope recognized by A300-934A maps to a region between residue 50 and 100 of human Geminin DNA replication Inhibitor using the numbering given in entry NP_056979.1 (GeneID 51053).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

IP, IHC

Product Type:
Antibodies
Alternative Names:
Gem, geminin, MGORS6
Species Reactivity:
Human
Immunogen:
Between 50 and 100
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Geminin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Geminin antibody A300-934A (lot A300-934A-2) used for IP at 3 µg per reaction. Geminin was also immunoprecipitated by rabbit anti-Geminin antibody A300-935A. For blotting immunoprecipitated Geminin, A300-935A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Geminin by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin (Cat. No. A300-934A Lot2) used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB

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