Antibody was affinity purified using an epitope specific to Coronin 1 immobilized on solid support. The epitope recognized by A300-932A maps to a region between residue 410 and the C-terminus (residue 461) of human Coronin 1 (Coronin, Actin Binding Protein 1A) using the numbering given in entry NP_009005.1 (GeneID 11151). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CLABP, CLIPINA, clipin-A, coronin, actin binding protein, 1A, coronin-1, coronin-1A, coronin-like protein A, coronin-like protein p57, HCORO1, IMD8, p57, TACO, tryptophan aspartate-containing coat protein
between 410 and C-term
2 - 8°C
Detection of human Coronin 1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Coronin 1 antibody A300-932A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Coronin 1 was also immunoprecipitated by rabbit anti-Coronin 1 antibodies A300-930A and A300-931A, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).