Antibody was affinity purified using an epitope specific to PRMT6 immobilized on solid support. The epitope recognized by A300-929A maps to a region between residue 325 and the C-terminus (residue 375) of human Protein Arginine Methyltransferase 6 using the numbering given in entry AAK85733.1 (GeneID 55170). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, histone-arginine N-methyltransferase PRMT6, HMT1 hnRNP methyltransferase-like 6, HRMT1L6, protein arginine N-methyltransferase 6
between 325 and C-term
2 - 8°C
Detection of human PRMT6 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PRMT6 antibody A300-929A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PRMT6 was also immunoprecipitated by rabbit anti-PRMT6 antibody A300-928A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human PRMT6 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PRMT6 (Cat. No. A300-929A Lot1) used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB