Antibody was affinity purified using an epitope specific to USP33 immobilized on solid support. The epitope recognized by A300-924A maps to a region between residue 425 and 475 of human Ubiquitin Specific Peptidase 33 using the numbering given in entry NP_055832.3 (GeneID 23032). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
deubiquitinating enzyme 33, hVDU1, pVHL-interacting deubiquitinating enzyme 1, ubiquitin carboxyl-terminal hydrolase 33, ubiquitin thioesterase 33, ubiquitin thiolesterase 33, ubiquitin-specific-processing protease 33, VDU1, VHL-interacting deubiquitinati
between 425 and 475
2 - 8°C
Detection of human USP33 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP33 antibody A300-924A used for IP at 3 µg/mg lysate. USP33 was also immunoprecipitated by rabbit anti-USP33 antibody A300-925A, which recognizes a downstream epitope. For blotting immunoprecipitated USP33, A300-925A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.