Antibody was affinity purified using an epitope specific to MCAK immobilized on solid support. The epitope recognized by A300-921A maps to a region between residue 50 and 100 of mouse Mitotic Centromere-Associated Kinesin using the numbering given in entry NP_608301.2 (GeneID 73804 ). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
4930402F02Rik, ESTM5, kinesin-like 6, kinesin-like protein KIF2C, Knsl6, MCAK, mitotic centromere-associated kinesin, X83316
between 50 and 100
2 - 8°C
Detection of mouse MCAK by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-MCAK antibody A300-921A used for WB at 0.1 µg/ml (A) and 1.0 µg/ml (B) and used for IP at 3 µg/mg lysate (B). MCAK was also immunoprecipitated by rabbit anti-MCAK antibody A300-922A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of mouse MCAK by immunohistochemistry. Sample: FFPE section of mouse hybridoma tumor. Antibody: Affinity purified rabbit anti-MCAK (Cat. No. A300-921A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB