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Rabbit anti-USP14 Antibody, Affinity Purified - A300-919A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-USP14 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-919A
Name:
Rabbit anti-USP14 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to USP14 immobilized on solid support.The epitope recognized by A300-919A maps to a region between residue 350 and 400 of human Ubiquitin Specific Peptidase 14 using the numbering given in entry NP_005142.1 (GeneID 9097).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
deubiquitinating enzyme 14, TGT, tRNA-guanine transglycosylase, 60-kD subunit, ubiquitin carboxyl-terminal hydrolase 14, ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase), ubiquitin specific protease 14 (tRNA-guanine transglycosylase), ubiqu
Species Reactivity:
Human
Immunogen:
Between 350 and 400
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human USP14 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T(50 µg) cells. Antibodies: Affinity purified rabbit anti-USP14 antibody A300-919A (lot A300-919A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human USP14 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP14 antibody A300-919A (lot A300-919A-2) used for IP at 6 µg/mg lysate. USP14 was also immunoprecipitated by a previous lot of this antibody (lot A300-919A-1) and rabbit anti-USP14 antibody A300-920A. For blotting immunoprecipitated USP14, A300-919A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human USP14 by immunohistochemistry. Samples: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti- USP14 (Cat. No. A300-919A Lot2 - left and Cat. No. A300-920A Lot1 - right) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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Detection of human USP14 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-USP14 (Cat. No. A300-919A Lot2 - left and Cat. No. A300-920A Lot1 - right) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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