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Rabbit anti-ASPP1 Antibody, Affinity Purified - A300-918A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-ASPP1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-918A
Name:
Rabbit anti-ASPP1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to ASPP1 immobilized on solid support.The epitope recognized by A300-918A maps to a region between residue 475 and 525 of human Apoptosis-Stimulated Protein of p53, 1 using the numbering given in entry NP_056131.1 (GeneID 23368).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
apoptosis-stimulating of p53 protein 1, apoptosis-stimulating protein of p53, 1, ASPP1, p53BP2-like, p85, Protein phosphatase 1 regulatory subunit 13B, protein phosphatase 1, regulatory (inhibitor) subunit 13B
Species Reactivity:
Human
Immunogen:
Between 475 and 525
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human ASPP1 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASPP1 antibody A300-918A (lot A300-918A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 60 seconds.

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Detection of human ASPP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASPP1 antibody A300-918A (lot A300-918A-2) used for IP at 6 µg per reaction. ASPP1 was also immunoprecipitated by rabbit anti-ASPP1 antibody A300-917A. For blotting immunoprecipitated ASPP1, A300-918A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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