Antibody was affinity purified using an epitope specific to KIF14 immobilized on solid support. The epitope recognized by A300-912A maps to a region between residue 1600 and the C-terminus (residue 1648) of human Kinesin Family Member 14 using the numbering given in entry NP_055690.1 (GeneID 9928). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
epididymis secretory sperm binding protein, kinesin-like protein KIF14, MCPH20, MKS12
between 1600 and C-term
2 - 8°C
Detection of human KIF14 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-KIF14 antibody A300-912A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). KIF14 was also immunoprecipitated by rabbit anti-KIF14 antibodies A300-232A and A300-233A, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Detection of human KIF14 by immunohistochemistry and immunocytochemistry. Sample: FFPE section of human breast carcinoma (left) and formaldehyde-fixed HeLa cells (right). Antibody: Affinity purified rabbit anti-KIF14 (Cat. No. A300-912A Lot1) used at a dilution of 1:200 (1µg/ml) and 1:100 (2µg/ml). Detection: DAB (left) and red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100 (right).