Antibody was affinity purified using an epitope specific to MTA1 immobilized on solid support. The epitope recognized by A300-911A maps to a region between residue 665 and the C-terminus (residue 715) of human Metastasis Associated 1 using the numbering given in entry NP_004680.1 (GeneID 9112). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
metastasis associated gene 1 protein, metastasis-associated protein MTA1
between 665 and C-term
2 - 8°C
Detection of human and mouse MTA1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTA1 antibody A300-911A (lot A300-911A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human MTA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTA1 antibody A300-911A (lot A300-911A-2) used for IP at 6 µg per reaction. MTA1 was also immunoprecipitated by a previous lot of this antibody (lot A300-911A-1). For blotting immunoprecipitated MTA1, A300-911A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.