Antibody was affinity purified using an epitope specific to Sin1 immobilized on solid support. The epitope recognized by A300-910A maps to a region between residue 470 and the C-terminus (residue 522) of human stress-activated map kinase interacting protein 1 using the numbering given in entry NP_001006618.1 (GeneID 79109). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
JC310, MEKK2-interacting protein 1, MIP1, mitogen-activated protein kinase 2-associated protein 1, mSIN1, ras inhibitor MGC2745, SAPK-interacting protein 1, SIN1, SIN1b, SIN1g, stress-activated map kinase interacting protein 1, Stress-activated map kinase
between 470 and C-term
2 - 8°C
Detection of human Sin1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 and 15 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sin1 antibody A300-910A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 7 minutes (A) and 30 seconds (B).