Rabbit anti-PPP3CA Antibody, Affinity Purified - A300-908A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to PPP3CA immobilized on solid support. The epitope recognized by A300-908A maps to a region between residue 470 and the C-terminus (residue 521) of human Protein Phosphatase 3, Catalytic Subunit, Alpha isoform using the numbering given in entry NP_000935.1 (GeneID 5530). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human and mouse PPP3CA by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP3CA antibody A300-908A (lot A300-908A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PPP3CA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP3CA antibody A300-908A (lot A300-908A-3) used for IP at 3 µg per reaction. PPP3CA was also immunoprecipitated by rabbit anti-PPP3CA antibody A300-907A. For blotting immunoprecipitated PPP3CA, A300-908A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PPP3CA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PPP3CA (Cat. No. A300-908A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
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