Antibody was affinity purified using an epitope specific to PPP1CC immobilized on solid support. The epitope recognized by A300-906A maps to a region between residue 280 and the C-terminus (residue 323) of human Protein Phosphatase 1, Catalytic Subunit, Gamma Isoform using the numbering given in entry NP_002701.1 (GeneID 5501). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
PP1C, PP-1G, PPP1G, protein phosphatase 1, catalytic subunit, gamma isoform, protein phosphatase 1, catalytic subunit, gamma isozyme, protein phosphatase 1C catalytic subunit, serine/threonine phosphatase 1 gamma, serine/threonine-protein phosphatase PP1-
between 280 and C-term
2 - 8°C
Detection of human PPP1CC by western blot and immunoprecipitation. Samples: Whole cell lysate (50, 15 and 5 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PPP1CC antibody A300-906A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PPP1CB was immunoprecipitated by rabbit anti-PPP1CB antibody A300-905A. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).