Antibody was affinity purified using an epitope specific to PPP1CB immobilized on solid support. The epitope recognized by A300-905A maps to a region between residue 280 and the C-terminus (residue 327) of human Protein Phosphatase 1, Catalytic Subunit, Beta Isoform using the numbering given in entry NP_996759.1 (GeneID 5500). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
epididymis secretory sperm binding protein Li 80p, HEL-S-80p, NSLH2, PP1B, PP-1B, PP1beta, PPP1CD, protein phosphatase 1, catalytic subunit, beta isoform, protein phosphatase 1, catalytic subunit, beta isozyme, protein phosphatase 1, catalytic subunit, de
between 280 and C-term
2 - 8°C
Detection of human PPP1CB by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (H, 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PPP1CB antibody A300-905A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PPP1CC was immunoprecipitated by rabbit anti-PPP1CC antibody A300-906A. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).