Antibody was affinity purified using an epitope specific to PPP1CA immobilized on solid support. The epitope recognized by A300-904A maps to a region between residue 280 and the C-terminus (residue 330) of human Protein Phosphatase 1, Catalytic Subunit, Alpha Isoform using the numbering given in entry NP_002699.1 (GeneID 5499). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
PP1A, PP-1A, PP1alpha, PPP1A, protein phosphatase 1, catalytic subunit, alpha isoform, protein phosphatase 1, catalytic subunit, alpha isozyme, serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit, serine/threonine-protein phosphatase PP1-al
between 280 and C-term
2 - 8°C
Detection of human and mouse PPP1CA by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP1CA antibody A300-904A (lot A300-904A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PPP1CA by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- PPP1CA (Cat. No. A300-904A Lot 3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB