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Rabbit anti-Pescadillo Antibody, Affinity Purified - A300-903A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Pescadillo Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-903A
Name:
Rabbit anti-Pescadillo Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to Pescadillo immobilized on solid support.The epitope recognized by A300-903A maps to a region between residue 535 and the C-terminus (residue 588) of human Pescadillo using the numbering given in entry NP_055118.1 (GeneID 23481).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
NOP7, PES, pescadillo homolog, pescadillo homolog 1, containing BRCT domain
Species Reactivity:
Human
Immunogen:
Between 535 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Pescadillo by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pescadillo antibody A300-903A (lot A300-903A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human Pescadillo by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pescadillo antibody A300-903A (lot A300-903A-2) used for IP at 3 µg per reaction. Pescadillo was also immunoprecipitated by rabbit anti-Pescadillo antibody A300-902A. For blotting immunoprecipitated Pescadillo, A300-903A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

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