Rabbit anti-Pescadillo Antibody, Affinity Purified - A300-902A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to Pescadillo immobilized on solid support. The epitope recognized by A300-902A maps to a region between residue 25 and 75 of human Pescadillo using the numbering given in entry NP_055118.1 (GeneID 23481). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
IP, IHC
Detection of human Pescadillo by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pescadillo antibody A300-902A used for IP at 3 µg/mg lysate. Pescadillo was also immunoprecipitated by rabbit anti-Pescadillo antibodies A300-903A and BL3962, which recognize downstream epitopes. For blotting immunoprecipitated Pescadillo, A300-903A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human Pescadillo by immunohistochemistry. Sample: FFPE sections of human colon carcinoma (left) and ovarian carcinoma (right). Antibody: Affinity purified rabbit anti- Pescadillo (Cat. No. A300-902A Lot1) used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB
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