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Rabbit anti-USP10 Antibody, Affinity Purified - A300-900A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-USP10 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-900A
Name:
Rabbit anti-USP10 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to USP10 immobilized on solid support.The epitope recognized by A300-900A maps to a region between residue 50 and 100 of human Ubiquitin Specific Peptidase 10 using the numbering given in entry NP_005144.1 (GeneID 9100).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
deubiquitinating enzyme 10, ubiquitin carboxyl-terminal hydrolase 10, ubiquitin specific protease 10, ubiquitin thioesterase 10, ubiquitin thiolesterase 10, ubiquitin-specific-processing protease 10, UBPO
Species Reactivity:
Human, Mouse
Immunogen:
Between 50 and 100
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human USP10 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP10 antibody A300-900A (lot A300-900A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of mouse USP10 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP10 antibody A300-900A (lot A300-900A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human USP10 by western blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP10 antibody A300-900A (lot A300-900A-2) used for IP at 3 µg per reaction. USP10 was also immunoprecipitated by rabbit anti-USP10 antibody A300-901A. For blotting immunoprecipitated USP10, A300-900A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human USP10 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- USP10 (Cat. No. A300-900A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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