Antibody was affinity purified using an epitope specific to USP28 immobilized on solid support. The epitope recognized by A300-899A maps to a region between residue 500 and 550 of human Ubiquitin Specific Peptidase 28 using the numbering given in entry NP_065937.1 (GeneID 57646). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
deubiquitinating enzyme 28, ubiquitin carboxyl-terminal hydrolase 28, ubiquitin carboxyl-terminal hydrolase 28 variant 1, ubiquitin thioesterase 28, ubiquitin thiolesterase 28, ubiquitin-specific-processing protease 28
between 500 and 550
2 - 8°C
Detection of human USP28 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP28 antibody A300-899A used for IP at 3 µg/mg lysate. USP28 was also immunoprecipitated by rabbit anti-USP28 antibodies A300-898A and BL3909, which recognize other epitopes. For blotting immunoprecipitated USP28, A300-898A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time 30 seconds.
Detection of human USP28 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- USP28 (Cat. No. A300-899A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB