Antibody was affinity purified using an epitope specific to USP28 immobilized on solid support. The epitope recognized by A300-898A maps to a region between residue 50 and 100 of human Ubiquitin Specific Peptidase 28 using the numbering given in entry NP_065937.1 (GeneID 57646). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
deubiquitinating enzyme 28, ubiquitin carboxyl-terminal hydrolase 28, ubiquitin carboxyl-terminal hydrolase 28 variant 1, ubiquitin thioesterase 28, ubiquitin thiolesterase 28, ubiquitin-specific-processing protease 28
between 50 and 100
2 - 8°C
Detection of human USP28 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (5, 15 and 50 µg for WB) and HeLa (1 mg for IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-USP28 antibody A300-898A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). USP28 was also immunoprecipitated by rabbit anti-USP28 antibodies A300-899A and BL3909, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).