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Rabbit anti-USP28 Antibody, Affinity Purified

Catalogue number:
A300-898A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to USP28 immobilized on solid support. The epitope recognized by A300-898A maps to a region between residue 50 and 100 of human Ubiquitin Specific Peptidase 28 using the numbering given in entry NP_065937.1 (GeneID 57646). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

deubiquitinating enzyme 28, ubiquitin carboxyl-terminal hydrolase 28, ubiquitin carboxyl-terminal hydrolase 28 variant 1, ubiquitin thioesterase 28, ubiquitin thiolesterase 28, ubiquitin-specific-processing protease 28

Species Reactivity:

Human

Immunogen:

between 50 and 100

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human USP28 by western blot

Detection of human USP28 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (5, 15 and 50 µg for WB) and HeLa (1 mg for IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-USP28 antibody A300-898A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). USP28 was also immunoprecipitated by rabbit anti-USP28 antibodies A300-899A and BL3909, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human USP28 by immunohistoc

Detection of human USP28 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- USP28 (Cat. No. A300-898A Lot1) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB