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Rabbit anti-JARID1A/RBP2 Antibody, Affinity Purified - A300-897A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-JARID1A/RBP2 Antibody, Affinity Purified
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Size: 20 µg
£302.00
GBP | Euro | USD
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Catalogue number:
A300-897A
Name:
Rabbit anti-JARID1A/RBP2 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to JARID1A/RBP2 immobilized on solid support.The epitope recognized by A300-897A maps to a region between residue 1640 and 1690 of human Jumonji/ARID Domain-Containing Protein 1A/Retinoblastoma Binding Protein 2 Homolog 1 using the numbering given in entry NP_001036068.1 (GeneID 5927).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
histone demethylase JARID1A, Jumonji, AT rich interactive domain 1A (RBP2-like), jumonji/ARID domain-containing protein 1A, lysine (K)-specific demethylase 5A, lysine-specific demethylase 5A, RBBP2, RBBP-2, RBP2, retinoblastoma binding protein 2, Retinobl
Species Reactivity:
Human, Mouse
Immunogen:
Between 1640 and 1690
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human JARID1A/RBP2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1A/RBP2 antibody A300-897A (lot A300-897A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of mouse JARID1A/RBP2 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1A/RBP2 antibody A300-897A (lot A300-897A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human JARID1A/RBP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1A/RBP2 antibody A300-897A (lot A300-897A-3) used for IP at 6 µg per reaction. JARID1A/RBP2 was also immunoprecipitated by a previous lot of this antibody (lot A300-897A-2). For blotting immunoprecipitated JARID1A/RBP2, A300-897A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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