Antibody was affinity purified using an epitope specific to JMJD2C immobilized on solid support. The epitope recognized by A300-885A maps to a region between residue 475 and 525 of human Jumonji Domain Containing 2C using the numbering given in entry NP_055876.2 (GeneID 23081). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
GASC1, GASC-1 protein, gene amplified in squamous cell carcinoma 1 protein, JHDM3C, JmjC domain-containing histone demethylation protein 3C, JMJD2C, jumonji domain-containing protein 2C, lysine (K)-specific demethylase 4C, lysine-specific demethylase 4C,
between 475 and 525
2 - 8°C
Detection of human JMJD2C by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-JMJD2C antibody A300-885A (Lot A300-885A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. JMJD2C was also immunoprecipitated by rabbit anti-JMJD2C antibody A300-885A (Lot A300-885A-1). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).