Antibody was affinity purified using an epitope specific to JMJD1C immobilized on solid support. The epitope recognized by A300-884A maps to a region between residue 150 and 200 of human Jumonji Domain Containing 1C using the numbering given in entry NP_004232.2 (GeneID 221037). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Jumonji domain-containing protein 1C, KDM3C, probable JmjC domain-containing histone demethylation protein 2C, thyroid hormone receptor interactor 8, thyroid receptor-interacting protein 8, TR-interacting protein 8, TRIP8, TRIP-8
between 150 and 200
2 - 8°C
Detection of human JMJD1C by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-JMJD1C antibody A300-884A used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human JMJD1C by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma. Antibody: Affinity purified rabbit anti- JMJD1C (Cat. No. A300-884A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB