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Rabbit anti-JMJD1B Antibody, Affinity Purified

Catalogue number:
A300-883A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to JMJD1B immobilized on solid support. The epitope recognized by A300-883A maps to a region between residue 1710 and the C-terminus (residue1761) of human Jumonji Domain Containing 1B using the numbering given in entry NP_057688.2 (GeneID 51780). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

5qNCA, C5orf7, jmjC domain-containing histone demethylation protein 2B, JMJD1B, jumonji domain containing 1B, jumonji domain-containing protein 1B, lysine (K)-specific demethylase 3B, lysine-specific demethylase 3B, NET22, nuclear protein 5qNCA

Species Reactivity:

Human

Immunogen:

between 1710 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human JMJD1B by western blo

Detection of human JMJD1B by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-JMJD1B antibody A300-883A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). JMJD1B was also immunoprecipitated by rabbit anti-JMJD1B antibody BL3820, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human JMJD1B by immunohisto

Detection of human JMJD1B by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- JMJD1B (Cat. No. A300-883A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB