Antibody was affinity purified using an epitope specific to JMJD1B immobilized on solid support. The epitope recognized by A300-883A maps to a region between residue 1710 and the C-terminus (residue1761) of human Jumonji Domain Containing 1B using the numbering given in entry NP_057688.2 (GeneID 51780). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
5qNCA, C5orf7, jmjC domain-containing histone demethylation protein 2B, JMJD1B, jumonji domain containing 1B, jumonji domain-containing protein 1B, lysine (K)-specific demethylase 3B, lysine-specific demethylase 3B, NET22, nuclear protein 5qNCA
between 1710 and C-term
2 - 8°C
Detection of human JMJD1B by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-JMJD1B antibody A300-883A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). JMJD1B was also immunoprecipitated by rabbit anti-JMJD1B antibody BL3820, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).