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Rabbit anti-CBX8 Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Wed 3 Apr
Shipping is calculated in checkout
Applications:

WB, IP, ChIP-Seq

Antibody Host:

Rabbit

Target:

CBX8

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 350 and C-term

Alternative Names:

Pc class 3 homolog; RC1; Polycomb 3 homolog; rectachrome 1; hPc3; chromobox protein homolog 8; chromobox homolog 8 (Pc class homolog, Drosophila); polycomb 3; PC3

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Product Description:

Rabbit anti-CBX8 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human CBX8 by western blot.
Detection of human CBX8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CBX8 antibody A300-882A (lot A300-882A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human CBX8 by western blot
Detection of human CBX8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CBX8 antibody A300-882A (lot A300-882A-2) used for IP at 6 µg per reaction. CBX8 was also immunoprecipitated by rabbit anti-CBX8 antibody BL3751. For blotting immunoprecipitated CBX8, A300-882A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Localization of CBX8 Binding Sites by Ch
Localization of CBX8 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CBX8 antibody A300-882A and analyzed by DNA sequencing. The figure illustrates the peak distribution of CBX8 binding within a 4 Mb region of chromosome 1 as detected using anti-CBX8 antibody A300-882A. ChIP-seq validation performed by Diogenode, Denville, NJ.
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