Antibody was affinity purified using an epitope specific to PPM1G immobilized on solid support. The epitope recognized by A300-881A maps to a region between residue 500 and the C-terminus (residue 546) of human Protein Phosphatase Magnesium-dependent 1 Gamma using the numbering given in entry NP_817092.1 (GeneID 5496). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
PP2CG, PP2CGAMMA, PP2C-gamma, PPP2CG, protein phosphatase 1C, protein phosphatase 1G, protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform, protein phosphatase 2, catalytic subunit, gamma isoform, protein phosphatase 2C gamma isoform,
between 500 and C-term
2 - 8°C
Detection of human and mouse PPM1G by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PPM1G antibody A300-881A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody A300-880A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).