Antibody was affinity purified using an epitope specific to PPM1G immobilized on solid support. The epitope recognized by A300-880A maps to a region between residue 400 and 450 of human Protein Phosphatase Magnesium-dependent 1 Gamma using the numbering given in entry NP_817092.1 (GeneID 5496). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
PP2CG, PP2CGAMMA, PP2C-gamma, PPP2CG, protein phosphatase 1C, protein phosphatase 1G, protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform, protein phosphatase 2, catalytic subunit, gamma isoform, protein phosphatase 2C gamma isoform,
between 400 and 450
2 - 8°C
Detection of human PPM1G by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PPM1G antibody A300-880A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody A300-881A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).
Detection of human and mouse PPM1G by immunohistochemistry. Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PPM1G (Cat. No. A300-880A Lot1) used at a dilution of 1:1,000 (0.2µg/ml) and 1:200 (1µg/ml). Detection: DAB