Antibody was affinity purified using an epitope specific to CBX5 immobilized on solid support. The epitope recognized by A300-877A maps to a region between residue 150 and the C-terminus (residue 191) of human Chromobox Homolog 5 using the numbering given in entry NP_036249.1 (GeneID 23468). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
antigen p25, chromobox homolog 5 (HP1 alpha homolog, Drosophila), chromobox protein homolog 5, epididymis luminal protein 25, HEL25, heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha, HP1, HP1 alpha, HP1 alpha homolog, HP1A, HP1-ALP
between 150 and C-term
2 - 8°C
Detection of human CBX5 by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBX5 antibody BL3740 (Cat. No. A300-877A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human CBX5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBX5 (Cat. No. A300-877A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB