Antibody was affinity purified using an epitope specific to EVI5 immobilized on solid support. The epitope recognized by A300-875A maps to a region between residue 760 and the C-terminus (residue 810) of human Ecotropic Viral Integrations Site 5 using the numbering given in entry NP_005656.4 (GeneID 7813). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
dJ846D11.1 (ecotropic viral integration site 5), ecotropic viral integration site 5 protein homolog, EVI-5, NB4S, neuroblastoma stage 4S gene protein
between 760 and C-term
2 - 8°C
Detection of human EVI5 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EVI5 antibody BL3342 (Cat. No. A300-875A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). EVI5 was also immunoprecipitated by rabbit anti-EVI5 antibodies BL3340 (Cat. No. A300-874A) and BL3341, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 1 second (B).