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Bromodomain-Containing (BRD) Antibodies

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Rabbit anti-EVI5 Antibody, Affinity Purified - A300-875A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-EVI5 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-875A
Name:
Rabbit anti-EVI5 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to EVI5 immobilized on solid support.The epitope recognized by A300-875A maps to a region between residue 760 and the C-terminus (residue 810) of human Ecotropic Viral Integrations Site 5 using the numbering given in entry NP_005656.4 (GeneID 7813).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
dJ846D11.1 (ecotropic viral integration site 5), ecotropic viral integration site 5 protein homolog, EVI-5, NB4S, neuroblastoma stage 4S gene protein
Species Reactivity:
Human
Immunogen:
Between 760 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human EVI5 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EVI5 antibody BL3342 (Cat. No. A300-875A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). EVI5 was also immunoprecipitated by rabbit anti-EVI5 antibodies BL3340 (Cat. No. A300-874A) and BL3341, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 1 second (B).

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