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Rabbit anti-EVI5 Antibody, Affinity Purified

Catalogue number:
A300-875A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to EVI5 immobilized on solid support. The epitope recognized by A300-875A maps to a region between residue 760 and the C-terminus (residue 810) of human Ecotropic Viral Integrations Site 5 using the numbering given in entry NP_005656.4 (GeneID 7813). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Detection of human EVI5 by western blot
Product Type:

Antibodies

Alternative Names:

dJ846D11.1 (ecotropic viral integration site 5), ecotropic viral integration site 5 protein homolog, EVI-5, NB4S, neuroblastoma stage 4S gene protein

Species Reactivity:

Human

Immunogen:

between 760 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human EVI5 by western blot

Detection of human EVI5 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EVI5 antibody BL3342 (Cat. No. A300-875A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). EVI5 was also immunoprecipitated by rabbit anti-EVI5 antibodies BL3340 (Cat. No. A300-874A) and BL3341, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 1 second (B).