Antibody was affinity purified using an epitope specific to EVI5 immobilized on solid support. The epitope recognized by A300-874A maps to a region between residue 525 and 575 of human Ecotropic Viral Integrations Site 5 using the numbering given in entry NP_005656.4 (GeneID 7813). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
dJ846D11.1 (ecotropic viral integration site 5), ecotropic viral integration site 5 protein homolog, EVI-5, NB4S, neuroblastoma stage 4S gene protein
between 525 and 575
2 - 8°C
Detection of human EVI5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EVI5 antibody BL3340 (Cat. No. A300-874A) used for IP at 3 µg/mg lysate. EVI5 was also immunoprecipitated by rabbit anti-EVI5 antibodies BL3341 and BL3342 (Cat. No. A300-875A), which recognize downstream epitopes. For blotting immunoprecipitated EVI5, BL3342 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.