Antibody was affinity purified using an epitope specific to RCC2 immobilized on solid support. The epitope recognized by A300-872A maps to a region between residue 1 and 50 of human Regulator of Chromosome Condensation 2 using the numbering given in entry NP_061185.1 (GeneID 55920). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
epididymis secretory sperm binding protein, protein RCC2, RCC1-like protein TD-60, TD-60, telophase disk protein of 60 kDa
between 1 and 50
2 - 8°C
Detection of human and mouse RCC2 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-RCC2 antibody BL3701 (Cat. No. A300-872A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).