Antibody was affinity purified using an epitope specific to SUPT5H immobilized on solid support. The epitope recognized by A300-869A maps to a region between residue 1040 and the C-terminus (residue 1087) of human Suppressor of Ty 5 Homolog using the numbering given in entry NP_003160.2 (GeneID 6829). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DRB sensitivity-inducing factor 160 kDa subunit, DRB sensitivity-inducing factor large subunit, DSIF large subunit, DSIF p160, hSPT5, SPT5, SPT5H, suppressor of Ty 5 homolog, Tat-cotransactivator 1 protein, Tat-CT1, Tat-CT1 protein, transcription elongati
between 1040 and C-term
2 - 8°C
Detection of human SUPT5H by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SUPT5H antibody BL3558 (Cat. No. A300-869A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B). SUPT5H was immunoprecipitated by rabbit anti-SUPT5H antibody BL3555 (Cat. No. A300-868A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).