Antibody was affinity purified using an epitope specific to SUPT5H immobilized on solid support. The epitope recognized by A300-868A maps to a region between residue 50 and 100 of human Suppressor of Ty 5 Homolog using the numbering given in entry NP_003160.2 (GeneID 6829). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DRB sensitivity-inducing factor 160 kDa subunit, DRB sensitivity-inducing factor large subunit, DSIF large subunit, DSIF p160, hSPT5, SPT5, SPT5H, suppressor of Ty 5 homolog, Tat-cotransactivator 1 protein, Tat-CT1, Tat-CT1 protein, transcription elongati
between 50 and 100
2 - 8°C
Detection of human SUPT5H by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUPT5H antibody BL3555 (Cat. No. A300-868A) used for IP at 3 µg/mg lysate. For blotting immunoprecipitated SUPT5H, rabbit anti-SUPT5H antibody BL3558 (Cat. No. A300-869A) was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.